Kuantifikasi Protein HAS2, PTX3, BCL2 dan BAX dalam Sel Kumulus Oosit Matur (MII) dan Imatur (MI) Pada Pasien Bayi Tabung

Oocyte developmental competence is one of the determining factors that in?uence outcomes an IVF cycle regarding ability a female gamete to reach maturation and be fertilized. A non-invasive approach using proteomic analysis oocyte cumulus cells (CCs) can used as method predict viability could potentially function molecular predictors for program prognosis. Our study was aimed at quantifying protein markers (HAS2, PTX3, BCL2 BAX) are expressed in human CCs between mature (MII) immature (MI) oocytes. This research analytic observational study. Twelve samples from oocytes were collected after ovum pick up patients undergoing ICSI. isolated ultrasonicator expressions quantified ELISA method. The Mann-Whitney test compare results showed HAS2, PTX3 proteins increase but had no significant effect compared oocytes, however, BAX expression significantly higher (P<0.05) which present shows quality maturation.
 Key words: ELISA, maturation, marker,